|
KMID : 1123920050190010212
|
|
Korean Journal of Oriental Physiology and Pathology
2005 Volume.19 No. 1 p.212 ~ p.217
|
|
|
Effects of Citrus Reticulata on the Cell Detachment and Apoptosis in Human Gastric Cancer SNU-668 Cells
|
|
Kim Jeung-Beum
Kim Min-Su
Kim E-Hwa
Kim Sung-Hoon
|
|
|
Abstract
|
|
|
|
The purpose of this study was to examine the effects of Citrus Reticulata(CR) on the Cell Detachment and Apoptosis in Human Gastric Cancer SNU-668 Cells. The effect of CR on apoptosis was investigated through MTT assay, DAPI staining, and TUNEL assay. We also performed RT-PCR for apoptotic genes including BCL-2, BAX, and caspase-3, the caspase-3 activity assay, and western blotting for pro-CASP-3. Then, to detect that adhesion of cell to ECM was reduced by CR, we investigated mRNA expression of CDH1 and PTK2 using RT-PCR, and their protein expressions using western blotting, and immunocytochemistry in SNU-668 cells. In this study, the results showed that treatment of CR induced time and dose-dependent cell death in SNU-668 cells. Downregulated mRNA expression of BCL-2, and upregulated mRNA expressions of BAX and CASP-3 indicated that the cell death was due to apoptosis. Protein expression of inactivated CASP-3, and caspase-3 activity assay also showed that apoptosis was induced in CR-treated cells.
|
|
|
KEYWORD
|
|
|
Citrus Reticulata(CR), Cell Detachment, Apoptosis, BCL-2, BAX, caspase-3
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|